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. 2000 Aug 7;150(3):667–680. doi: 10.1083/jcb.150.3.667

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Immunofluorescence of WISH cultures using the mAb201 (α-hFbn) antibody (a), Tsk/Tsk cultures using the pAb9543 (α-Fbn) antibody (b), and WISH and Tsk/Tsk cocultures using the pAb9543 (α-Fbn) (c), pAb8368 (α-Tsk) (d and g), or mAb201 (α-hFbn) antibody (e and h). f and i show the superimposition of the images in d and e and g and h, respectively. The antibody pAb9543 (α-Fbn) was employed to better appreciate the morphology (b) and extent (c) of the microfibrillar aggregates; additionally, nuclei of cocultured cells were stained with propidium iodine to document the level of cell confluency. Bars: (a and b) 20 μm; (c) 60 μm; (d–i) 40 μm.