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. 2000 Aug 7;150(3):667–680. doi: 10.1083/jcb.150.3.667

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Electron microscopy of fibrillin 1 microfibrils extracted from the matrix of cocultures of WISH and Tsk/Tsk fibroblasts and viewed after rotary shadowing (a and b) or after negative staining (c–f). Arrows in a indicate abnormal globular morphologies. Arrowheads in d indicate irregularities in periodicity. Microfibrils in e and f are labeled with mAb201 (recognized by the large, 10-nm, gold conjugate), specific for human fibrillin 1, and pAb8368 (recognized by the small, 5-nm, gold conjugate), specific for mouse Tsk fibrillin 1. Bar, 100 nm.