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. 1998 Dec 28;143(7):1775–1787. doi: 10.1083/jcb.143.7.1775

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bub1 ⁺ function is essential for high fidelity chromosome transmission through mitosis. (A) The diploid state can not be maintained in the absence of a functional bub1 ⁺ gene. Diploids were forced by intragenic complementation between the red (ade6-210) and pink (ade6-216) alleles of the ade6 locus. Ade⁺ cells were harvested from medium lacking adenine and plated onto YES 1/10 ade medium to allow the development of red and pink colors. The bub1 ⁺/bub1 ⁺ (wild-type) diploid was stable, producing pure white colonies whereas the Δbub1/Δbub1 strain readily broke down to the haploid state, producing red, pink, and sectored colonies. (B) Chromosomes lag on the anaphase spindle in Δbub1 cells. Exponential growing cells in YES medium at 26°C were fixed and processed for tubulin (green) and DAPI (red, pseudocolor) staining. Right: In wild-type haploid and diploid anaphases, the chromatin masses are at the spindle poles. Left: Δbub1 haploid and diploid cells with chromosomes remaining in the midzone of the anaphase spindle. Bar, 10 μm.