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. 1998 Dec 28;143(7):1775–1787. doi: 10.1083/jcb.143.7.1775

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Bub1 localizes at kinetochores in nda3KM311-arrested cells. Cells from strain 415 (nda3KM311 bub1-HA) were grown to early log phase at 32°C and shifted to 18°C for 8 h. Fixed cells were stained with mHA (red) and centromeric DNA was detected by FISH (green). Blue, DAPI staining. Bottom right, merged image. Two cells are shown. In some instances, Bub1 staining produces three doublets (the cell at the bottom). The merged image reveals that centromeric DNA is located precisely in between the paired dots, consistent with Bub1 being located on each sister kinetochore. Bar, 10 μm.