Table II.
Loss of Sister Chromatid Cohesion in nda3-KM311–arrested Δbub1 Cells
| Strain | Background | Time at 18°C | Number of cells examined | Number of cells with 1, 2, 3, and >3 cenFISH signals* | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | >3 | |||||||||||
| h | ||||||||||||||
| 1 | 418 | 399 | 16 | 3 | 0 | |||||||||
| 379 | nda3KM311 | 5 | 421 | 273 | 115 | 33 | 0 | |||||||
| 15 | 422 | 179 | 179 | 64 | 0 | |||||||||
| 1 | 431 | 397 | 30 | 3 | 1 | |||||||||
| 401 | nda3KM311 Δbub1 | 5 | 428 | 300 | 71 | 42 | 15 | |||||||
| 15 | 408 | 241 | 96 | 46 | 25 | |||||||||
One cenFISH signal was defined as a well-individualized spot of fluorescence; doublets were scored as a single signal.