Table V.
Frequency of Abnormal Anaphases in Δbub1 Cells upon Depletion of Swi6
| Strain | Relevant background | Swi6* | Number of late-anaphase cells analyzed‡ | Late-anaphase cells with lagging chromosomes§ | ||||
|---|---|---|---|---|---|---|---|---|
| + | 203 | 1 (0.5%) | ||||||
| 57 | nmtswi6, bub1 + | |||||||
| − | 300 | 8 (2.7%) | ||||||
| + | 202 | 15 (7.4%) | ||||||
| 399 | nmtswi6, Δbub1 | |||||||
| − | 100 | 50 (50%) |
*
Cells were grown to early log phase at 32°C on minimal medium without thiamine (Swi6 +), then thiamine was added and cells grown for 8–10 generations (Swi6 −). Samples were fixed and processed for tubulin and chromatin staining.
‡
Late-anaphase cells were defined as cells with a spindle length greater than 5 μm.
§
Cells were classified as having lagging chromosomes when DAPI-stained material was observed on the spindle, at least 1.5 μm away from one spindle pole. Parentheses, fraction of anaphase cells with lagging chromosomes.