Table I.
Yeast Strain List
| Strain* | Genotype | |
|---|---|---|
| ProA5-4d/4a | MAT a/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Δ200/his3Δ200 trp1-1/trp1-1 | |
| leu2-3,112/leu2-3,112 lys2-801/lys2-801 | ||
| ProA5-4d | MAT a NDC1-ProA-HIS3-URA3 ura3-52 his3Δ200 trp1-1 leu2-3,112 lys2-801 | |
| HC26-15a/1a | MAT a/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1::URA3/nup49-1::URA3 nup49ΔGFLG::GFP- | |
| S65T-TRP1/nup49ΔGFLG::GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 | ||
| HC27-16d/16b | MAT a/MATα nup133Δ::HIS3/nup133Δ::HIS3 NDC1-ProA-HIS3-URA3/NDC1 nup49-1::URA3/nup49-1::URA3 | |
| nup49ΔGFLG::GFP-S65T-TRP1/nup49ΔGFLG::GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 | ||
| HC22-2b/1c | MAT a/MATα NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 spc42Δ::LEU2/spc42Δ::LEU2 | |
| TRP1::SPC42-GFP(3x)/TRP1::SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 | ||
| HC23-11d/16a | MAT a/MATα NIC96-ProA-HIS3-URA3/NIC96-ProA-HIS3-URA3 spc42Δ::LEU2/spc42Δ::LEU2 TRP1::SPC42- | |
| GFP(3x)/TRP1::SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 | ||
| HC14-10c(1198)/HC29-6b | MAT a/MATα ndc1Δ::KANMX/ndc1Δ::HIS3 ura3-52/ura3-52 his3Δ200/his3Δ200 trp1Δ63/TRP1 leu2Δ1/leu2Δ1 LYS2/ | |
| lys2-801 ade2Δ426/ade2Δ426 ade3Δ/ade3Δ (pRS315-NDC1-GFP) | ||
| HC10-42b/42c | MAT a/MATα ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 | |
| HC10-42a/42d | MAT a/MATα ndc1-1/ndc1-1 ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 | |
| HC13-1c | MATα NIC96-ProA-HIS3-URA3 ura3-52 his3Δ200 trp1-1 leu2-3,112 lys2-801 | |
| Wx340-3c | MATα ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 | |
| Wx340-3d | MATα tub2-403 ura3-52 his4-539 | |
| HC14-10c | MAT a ura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ | |
| HC5-31c | MAT a ndc1-1 ura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ | |
| HC5-31c(1166) | MAT a ndc1-1 pom152Δ::HIS3 ura3-52 his3Δ200 trp1Δ63 leu2Δ1 ade2Δ426 ade3Δ |
*
All strains were constructed as a part of this study.