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. 1998 Dec 28;143(7):1831–1844. doi: 10.1083/jcb.143.7.1831

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Experimental design of the ISG fusion assay. (a) Processing of SgII by PC2. The major sulfated processing products of SgII (38, 24, and 18 kD) are illustrated with the full-length protein (86 kD). Solid boxes, sequences recognized by the antibodies used in this study; arrowheads, the di-basic amino acid sites used by PC2; solid dots, the tyrosine residue which is sulfated by TPST. (b) Scheme of ISG– ISG fusion assay. *, [³⁵S]sulfate-labeled proteins. See Materials and Methods for details. (c) Immunoprecipitation with ab100, ab175, and ab69 using lysates obtained from [³⁵S]sulfate-labeled PC12/PC2 cells (lanes 1–3) or PC12 cells (lane 4). Arrows, position of SgII (p86), and the processing products p38, p24, and p18.

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