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. 1998 Dec 28;143(7):1831–1844. doi: 10.1083/jcb.143.7.1831

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Fusion occurs with enriched acceptor ISGs. A membrane pellet (MP) or velocity gradient (VG) fraction (pool of fractions 2–4) were isolated from a PNS obtained from PC12 cells labeled with [³⁵S]sulfate for 30 min. A standard reaction was performed with or without 10 μl of PC2-ISGs and with or without rat liver cytosol. After a 30-min incubation for fusion, processing was performed for 90 min at 37°C. p18 was immunoprecipitated and quantitated as above. Data shown is of representative experiment performed in triplicate (error bars are ±SD of the mean) expressed as percent of the control. For comparison, the PC2-dependent signal is shown relative to the PC2-independent signal as an inset to emphasize that ISG–ISG fusion is comparable in the MP and VG fractions.