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. 1998 Dec 28;143(7):1871–1881. doi: 10.1083/jcb.143.7.1871

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Immunofluorescence localization of endogenous dyn2 in HeLa cells. tTA-HeLa cells were processed for indirect immunofluorescence as described in Materials and Methods. Shown is the punctate localization of endogenous dyn2, as detected using the mAb Hudy-1 (a and b), its colocalization with puncta located at the cell periphery detected using anti-clathrin light chain rabbit sera (a′) and the lack of colocalization with the human TGN48, detected using monospecific rabbit sera (b′). Secondary antibodies were Alexa 488-conjugated goat anti–mouse and Texas red– conjugated goat anti–rabbit. Images were obtained using a Zeiss Axiovert 100TV, with the Bio-Rad MRC1024 confocal system and show a projection of either 4 optical sections taken through the middle of the cell (a and a′) or of 12 optical sections from the bottom to top of the cell (b and b′).