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. 1998 Dec 28;143(7):1813–1830. doi: 10.1083/jcb.143.7.1813

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Cellular distribution of Kap121-GFP and Nmd5-GFP in nup53Δ and nup59Δ null mutants. A plasmid-born copy of the Kap121-GFP or Nmd5-GFP chimeric gene was introduced into four haploid yeast strains: wild-type DF5, nup53Δ (NP53-B1), nup59Δ (NP59-23), nup53Δnup59Δ (NP53/NP59-2.1), and, for Kap121-GFP, the nup53Δnup59Δ strain containing a plasmid-born copy of NUP53 (pRS315-NUP53). In each case, cells were grown to mid-log phase and examined directly by fluorescent microscopy. Similarly, the distribution of GFP-Nup49p was examined in wild-type DF5 and nup53Δnup59Δ strains. Immunofluorescence microscopy was also performed on these strains using mAb414 to detect the localization of FXFG-containing nucleoporins. The position of the nuclear DNA is visualized by DAPI staining. Bar, 5 μm.