Table I.
Yeast Strains
| Strains | Genotype | Derivation | ||
|---|---|---|---|---|
| W303 | Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 | see Aitchison et al., 1995 | ||
| trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 | ||||
| DF5 | Mata/Matα ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-1/trp1-1 | |||
| leu2-3,112/leu2-3,112 lys2-801/lys2-801 | ||||
| NP53Δ | Mata/Matα ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-1/trp1-1 | see text | ||
| leu2-3,112/leu2-3,112 lys2-801/lys2-801 nup53::HIS3/+ | ||||
| NP53-B1 | Mata ura3-52 his3-Δ200 trp1-1 leu2-3,112 lys2-801 nup53::HIS3 | Segregant of sporulated NP53Δ | ||
| NP53-A2 | Matα ura3-52 his3-Δ200 trp1-1 leu2-3,112 lys2-801 nup53::HIS3 | Segregant of sporulated NP53Δ | ||
| NP59Δ | Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 | see text | ||
| trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 nup59::HIS3/+ | ||||
| NP59-21 | Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup59::HIS3 | Segregant of sporulated NP59Δ | ||
| NP59-23 | Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup59::HIS3 | Segregant of sporulated NP59Δ | ||
| NP53/NP170-11 | ade2 ura3 his3 trp1 leu2 nup53::HIS3 nup170::HIS3 | Segregant of sporulated NP53/NP170 | ||
| pRS316A-NUP170 (ADE3 URA3) | containing pRS316A-NUP170 | |||
| NP59/NP170-7c | ade2 ura3 his3 trp1 leu2 can1 nup59::HIS3 nup170::HIS3 | Segregant of sporulated NP59/NP170 | ||
| pRS316A-NUP170 (ADE3 URA3) | containing pRS316A-NUP170 | |||
| NP53/NP188-6a | ura3 his3 trp1 leu2 nup53::HIS3 nup188::HIS3 pRS316A-NUP188 | Segregant of sporulated NP53/NP188 | ||
| (ADE3 URA3) | containing pRS316A-NUP188 | |||
| NP59/NP188-7a | ade2 ade3 ura3 his3 trp1 leu2 can1 nup59::HIS3 nup188::HIS3 | Segregant of sporulated NP59/NP188 | ||
| pRS316A-NUP59 (ADE3 URA3) | containing pRS316A-NUP159 | |||
| NP53/NP157-77a | ura3 his3 trp1 leu2 nup53::HIS3 nup157::URA3 | Segregant of sporulated NP53/NP157 | ||
| NP59/NP157-π3 | ade3 ura3 his3 trp1 leu2 nup59::HIS3 nup157::URA3 | Segregant of sporulated NP59/NP157 | ||
| NP59/PM152-AC | ade2 ura3 his3 trp1 leu2 can1 nup59::HIS3 pom152::HIS3 | Segregant of sporulated NP59/PM152 | ||
| pRS316A-NUP59 (ADE3 URA3) | containing pRS316A-NUP59 | |||
| NP53/NP59-2.1 | ade2 ura3 his3 trp1 leu2 nup53:HIS3 nup59::HIS3 | Segregant of sporulated NP53/NP59 | ||
| NP53PA | Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 | Integrative transformation of W303 | ||
| trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 | with Protein A-HIS3-URA3 at the | |||
| nup53-pA/+ (URA3 HIS3) | 3′ end of NUP53 | |||
| NP53pPA | Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 | W303 haploid transformed with | ||
| pRS315-NUP53-pA (LEU2) | pRS315-NUP53-pA | |||
| NP59pPA | Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 | NP59-21 transformed with pRS315-NUP59-pA | ||
| nup59::HIS3 pRS315-NUP59-pA (LEU2) | ||||
| cdc15-2 | Mata ade1 ade2 ura1 his7 lys2 tyr1 gal1 cdc15-2 | see Hartwell, 1971 | ||
| cdc15-2B | Matα cdc15-2 | Segregant derived from cross of cdc15-2 with PSE1-A | ||
| cdc15-2-53 | Matα cdc15-2 pRS315-NUP53-pA (LEU2) | cdc15-2B transformed with pRS315-NUP53-pA | ||
| cdc15-2-59 | Matα cdc15-2 pRS315-NUP59-pA (LEU2) | cdc15-2B transformed with pRS315-NUP59-pA | ||
| cdc15/cdc15-2B | Mata/Matα cdc15-2/cdc15-2 | Cross of cdc15-2 and cdc15-2B | ||
| cdc15/cdc15-2B-53 | Mata/Matα cdc15-2/cdc15-2 pRS315-NUP53-pA (LEU2) | cdc15-2B transformed with pRS315-NUP59-pA | ||
| cdc152/cdc15-2B-121 | Mata/Matα cdc15-2/cdc15-2 pPS1069 (TRP1) | cdc15-2B transformed with pPS1069 (KAP121-GFP) | ||
| NP53/KP123 | ura3-52 his3-Δ leu2-3,112 lys2-801 kap123::URA3 nup53::HIS3 | Segregant derived from cross of | ||
| NP53-A2 and 123Δ-14-1 (Rout et al., 1997) | ||||
| NP53/KP123 | ura3-52 his3-Δ leu2-3,112 lys2-801 kap123::URA3 nup53::HIS3 | Segregant derived from cross of NP59-21 and | ||
| 123Δ-14-1 (Rout et al., 1997) |