Table 1.
Immunohistochemical, RT-PCR, and Flow Cytometry Analysis of pp6, mc13, MD1, MD2, and Fibroblasts
| pp6 | mc13 | MD1 | MD2 | Fibroblasts | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Immuno | RT-PCR | Flow | Immuno | RT-PCR | Flow | Immuno | RT-PCR | Flow | Immuno | RT-PCR | Flow | Immuno | RT-PCR | Flow | |
| Desmin | + | nd | nd | + | nd | nd | + | nd | nd | + | nd | nd | − | nd | nd |
| CD34 | + | + | nd | − | − | nd | + | + | nd | + | + | nd | − | − | nd |
| Bcl-2 | + | nd | nd | +/− | nd | nd | nd | − | nd | nd | − | nd | − | nd | nd |
| Flk-1 | + | nd | nd | + | nd | nd | + | nd | nd | + | nd | nd | − | nd | nd |
| Sca-1 | + | nd | + | + | nd | + | + | nd | + | + | nd | + | − | nd | − |
| M-cadherin | −/+ | nd | nd | +/− | nd | nd | − | nd | nd | − | nd | nd | − | nd | nd |
| Myogenin | −/+ | + | nd | +/− | + | nd | nd | + | nd | nd | + | nd | − | − | nd |
| C-met | nd | + | nd | nd | + | nd | nd | + | nd | nd | + | nd | nd | − | nd |
| MNF | nd | + | nd | nd | + | nd | nd | + | nd | nd | + | nd | nd | − | nd |
| MyoD | +/− | + | nd | nd | + | nd | nd | + | nd | nd | + | nd | nd | − | nd |
| C-kit | nd | nd | − | nd | nd | − | nd | nd | − | nd | nd | − | nd | nd | − |
| CD45 | nd | nd | − | nd | nd | − | nd | nd | − | nd | nd | − | nd | nd | − |
+, >95%; −, <2%; +/−, 40–80% of cells in the culture expressed the antigen; −/+, 5–30% of cells expressed the antigen; nd, not determined.