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. 2000 Sep 4;150(5):1057–1070. doi: 10.1083/jcb.150.5.1057

Figure 2.

Figure 2

Activation of gp60 using cross-linking Ab increases vesicle formation in endothelial cells. BPMVEC were incubated with either anti-gp60 Ab (plus secondary Ab) or control Ab (preimmune IgG; 5 μg/ml) for 30 min at 4°C. Cells were incubated at 37°C for 15 min with 5 μg/ml FM 1-43 plus 10 mg/ml BSA. Median brightness values of cells and the number of fluorescent particles per cell were determined after three washes with buffer. The asterisk indicates increased particle density and median cell brightness (P < 0.05) from corresponding control values.