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. 2000 Sep 4;150(5):1057–1070. doi: 10.1083/jcb.150.5.1057

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effects of caveolin-1 overexpression and dominant negative Src (dn-Src) expression on the association of G_αi and G_αq with caveolin-1. BLMVEC transfected with pcDNA3.1 alone (Mock); and wt-caveolin-1 (cav-1) or dn-Src was grown to confluence (48 h posttransfection). Cells were washed and lysed (see details in Materials and Methods), and the lysate was immunoprecipitated using 2.5 μg/ml anti–caveolin-1 Ab. Precipitated proteins were separated by SDS-PAGE and transferred to the Duralose membrane. The membrane was blotted with anti–caveolin-1 Ab (top), anti-G_αi Ab (middle), and anti-G_αq Ab (bottom). Representative data from three experiments are shown.