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. 2000 Sep 4;150(5):1189–1198. doi: 10.1083/jcb.150.5.1189

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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MacroH2A1 expression and intracellular location in undifferentiated male and female ES cells. a, MacroH2A1 localizes to centrosomes in interphase J1 male ES cells and 2-1 female ES cells. Centrosomes were marked with anti–γ-tubulin immunostaining and macro H2A1 was identified with an affinity-purified polyclonal antibody (Costanzi and Pehrson 1998). b, macroH2A1 remains associated with centrosomes in mitotic ES cells. Metaphase J1 ES cells stained with antibodies specific for either γ-tubulin or α-tubulin and antimacroH2A1 antibodies. c, Antimacro H2A1 immunoblot of total protein extracted from undifferentiated male J1 ES cells.