Figure 8.

Disassembly of actin filaments dissociates between the Sec6/8 complex and Ca²⁺ signaling proteins. Brain extract was used to immunoprecipitate proteins with anti-Sec8 Abs. After IP, the Sepharose A beads were divided into two portions. One portion was treated with buffer (con) and one portion with 20 μg/ml of the NH₂-terminal fragment of gelsolin (Gelsol) for 20 min at 0°C. The beads were then washed before analysis of proteins remaining attached to the beads by Western blots. Note that Sec6 remained bound to Sec8, but actin and all Ca²⁺ signaling proteins were released as a consequence of actin disassembly.