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. 2000 Sep 4;150(5):1161–1176. doi: 10.1083/jcb.150.5.1161

Figure 10.

Figure 10

l-Afadin–dependent recruitment of E-cadherin to nectin-based cell–cell adhesion sites without the trans-interaction of E-cadherin. Nectin-1α-L cells were cocultured with nectin-1α-EL or -1α-ΔC-EL cells in the presence or absence of gD and double stained with the anti–nectin-1α Ab1 and the anti–E-cadherin Ab. In the absence of gD, E-cadherin was concentrated at ∼40% of the adhesion sites between nectin-1α-L and -1α-EL cells. The recruitment of E-cadherin was observed in ∼20% of the adhesion sites between nectin-1α-L and -1α-ΔC-EL cells. In the presence of gD, the recruitment of E-cadherin was observed in ∼8% of the adhesion sites between nectin-1α-L and -1α-EL cells. (arrows) Cell–cell adhesion sites between two nectin-1α-L cells or between two nectin-1α-ΔC-L cells; (arrowheads) cell–cell adhesion sites between nectin-1α-L and -1α-EL or -1α-ΔC-EL cells; and (double arrowheads) cell–cell adhesion sites between two nectin-1α-EL cells or between two nectin-1α-ΔC-EL cells. The results shown are representative of three independent experiments.