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l-Afadin–dependent recruitment of α-catenin to nectin-based cell–cell adhesion sites in the absence of E-cadherin. Nectin-2α-L and -2α-ΔC-L cells were transfected with pPGKIZ-HA-α-catenin and double stained with the anti–nectin-2 and anti-HA Abs. (arrow) Nectin-2α–based cell–cell adhesion sites; and (arrowhead) nectin-2α-ΔC–based cell–cell adhesion sites. The results shown are representative of three independent experiments. Bars, 10 μm.
