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. 2000 Sep 4;150(5):963–974. doi: 10.1083/jcb.150.5.963

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Early nocodazole treatment delays MPF activation. Western blots (details and annotations as in the legend to Fig. 2) showing the behavior of Cdc2, Cdc25C, and cyclin B2 during first mitosis after treatment at different times with 10 mM nocodazole. Treatments were started at 0.26, 0.44, and 0.66 NT as indicated. The times below each lane show the period of collection and freezing of control and treated eggs. Eggs treated at 0.26 NT showed a delay in Cdc2 and Cdc25C activation of ∼0.1 time units, whereas treatments beginning at 0.44 and 0.66 NT did not. Degradation of cyclin B2 was delayed in the early nocodazole-treated eggs and appeared to degrade more slowly.