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. 2000 Sep 4;150(5):1209–1214. doi: 10.1083/jcb.150.5.1209

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Costameric γ-actin is appropriately expressed and localized in mdx muscle in situ. Shown in a–c are images of sarcolemma from normal mouse muscle stained with antibodies specific to α-sarcomeric (a), β-nonmuscle (b), or γ-actin (c). Shown in d and e are images of control (d), or mdx sarcolemma (e) double stained with monoclonal antibodies to α-actinin (green) and polyclonal antibodies to γ-actin (red). Areas of coincidence between the two probes appear yellow. Shown in f and g are images of fixed and permeabilized myofibers from control (f) and mdx (g) muscle stained with antibodies specific for γ-actin. Bars, 10 μm.