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. Author manuscript; available in PMC: 2008 Dec 7.
Published in final edited form as: J Mol Biol. 2007 Oct 10;374(4):936–950. doi: 10.1016/j.jmb.2007.10.005

Figure 7.

Figure 7

By Western blotting, UNC-94 polypeptides can be detected from wild-type, but are absent from or in reduced amounts in unc-94 mutants or RNAi animals. (a) Affinity-purified anti-UNC-94 antibodies detect proteins of the expected size (~45 kDa) for the products of the unc-94 gene from wild type C. elegans. Note that these bands are absent from unc-94(sf20) and unc-94(RNAi) animals (“Empty vector” refers to the use of the RNAi feeding vector without insert). (b) The same blot was washed and then reacted with anti-paramyosin to demonstrate equal loading of total protein in each lane. The columns of numbers and their positions represent molecular weight markers in kDa.