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Microinjection of M130Ab induces stress fiber formation and increases MLC phosphorylation in serum-starved 3T3 cells. M130Ab (5 mg/ml) was microinjected into serum-starved 3T3 cells. FITC-dextran was coinjected to identify injected cells (b, d, and f). 2 h after injection, cells were fixed and stained with rhodamine-phalloidin (a–d), or anti–S19-phosphorylated MLC antibody (e and f). Asterisks indicated uninjected cells. Bars, 10 μm.
