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. 2000 Aug 21;150(4):797–806. doi: 10.1083/jcb.150.4.797

Figure 5.

Figure 5

Effects of ROCK or MLCK inhibitors on M130Ab-induced stress fiber and focal adhesion assembly. Serum-starved 3T3 cells were first treated for 30 min with 10 μM Y-27632 alone (a–f), 10 μM ML-9 alone (g and h) or both 10 μM Y-27632 and 10 μM ML-9 (i and j). M130Ab (5 mg/ml) was then microinjected together with FITC-dextran. 2 h after injection, cells were fixed and stained with rhodamine-phalloidin (a, b, and g–j), anti S19-phosphorylated MLC antibody (c and d), or anti-vinculin antibody (e and f). In k–n, 3T3 cells grown with 10% serum were pretreated with 10 μM Y-27632 (k and l) or 10 μM ML-9 (m and n) for 30 min, and microinjected with M130Ab. 2 h after injection, cells were fixed and stained with rhodamine-phalloidin. Injected cells were detected by coinjected FITC-dextran (b, d, f, h, j, l and n). Uninjected cells are indicated by asterisks. Bars, 10 μm.