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. 2000 Aug 21;150(4):861–872. doi: 10.1083/jcb.150.4.861

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The amount of ILK in Triton X-100–soluble fractions is reduced during terminal myogenic differentiation. Mouse C2C12 myoblasts were cultured in growth medium (GM) and induced to differentiate by switching to the differentiation medium (DM). The cells were extracted with lysis buffers containing 1% Triton X-100 or 1% SDS as described in Materials and Methods. ILK in the Triton X-100 (lanes 1 and 2) or SDS (lanes 3 and 4) extracts were analyzed by immunoblotting with a monoclonal anti-ILK antibody 65.1.