Figure 2.

Calreticulin modulates dexamethasone-dependent apoptosis of A1.1 cells. (A) Western blot analysis of calreticulin in A1.1 cells incubated with calreticulin-specific anti-sense oligodeoxynucleotide. Cells were lysed with RIPA buffer, protein separated in SDS-PAGE, transferred to nitrocellulose membrane, and probed with goat anti-calreticulin antibodies. Lanes 1–3, 10, 20, and 30 μg of cell extract from anti-sense oligodeoxynucleotide-treated A1.1 cells, respectively; lanes 4–6, 10, 20, and 30 μg of cell extract from untreated A1.1 cells, respectively. CRT, calreticulin. (B) Downregulation of expression of calreticulin was regulated in A1.1 cells by incubation with specific anti-sense oligodeoxynucleotide followed by DNA fragmentation analysis. Apoptosis was induced with a different concentration of Dex. Open bar, control cells; hatched bar, cells treated with scrambled control oligodeoxynucleotide; full bar, cells treated with anti-sense oligodeoxynucleotide. Data are means ± SD of three independent experiments.