Figure 4.
Thapsigargin-dependent induction of apoptosis in cells overexpressing calreticulin and calnexin. KN1 and KNX2 cells were incubated with 2 μg Dox/ml (filled bars) for 24 h to induce expression of calreticulin and calnexin, respectively. Cells were treated with thapsigargin followed by Annexin-V binding assay. HeLa-On, mock transfected Tet-On HeLa cells. (Open bars) Cells not incubated with Dox; filled bars, Dox-treated cells. 100% value corresponds to 10,000 cells. Data are means ± SD of three independent experiments. ** P < 0.001.