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. 2000 Aug 21;150(4):929–936. doi: 10.1083/jcb.150.4.929

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Whole-mount photographs of 16–32-cell stage embryos, coinjected with LY (M _r = 457) and dextran-rhodamine (M _r = 10,000). Dextran is too large to pass through gap junctional channels and serves as a marker for the injected cell and all cells joined to the injected cell by cytoplasmic bridges. A and B show examples of undetectable dye transfer, while C and D show a sample of embryos where LY appears to spread away from the injected cell. Notice, however, that the nonpermeant dextran shows an identical pattern to the LY. Bar, 0.5 mm.