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. 2000 Aug 21;150(4):929–936. doi: 10.1083/jcb.150.4.929

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intraembryonic reflection and selective masking of fluorescent signal by pigmented cells in whole-mount embryos. 32-cell stage embryos were injected with dextran-fluorescein (DF, mol wt = 10,000) in one dorsal (less pigmented, A) or one ventral (highly pigmented, B) animal cell in tier 1. The lens effect is demonstrated in whole-mount A where fluorescence is seen in many surrounding cells. In contrast, only two less intensely fluorescent cells are seen in whole-mount B. However, sections of embryo A in C and of embryo B in D confirm that after either injection, the junction-impermeable dextran-fluorescein is confined to the injected cell and to those cells that are joined by cytoplasmic bridges. Bars: (A) 250 μm; (C) 200 μM.