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. 2007 Oct 16;35(20):7011–7022. doi: 10.1093/nar/gkm724

Figure 5.

Figure 5.

Identification of an hTERT fragment (EID) that binds hEST1A. (A) Schematic representation of full-length hTERT and hTERT fragments. The different hTERT domains are indicated (32). For each polypeptide the molecular weight is given in kDa and the binding of hEST1A as determined in B is indicated (+, binding; −, no binding and ?, not known). (B) Transient co-expression in 293T cells of FLAG-hEST1A and full-length hTERT, full length Myc-hTERT (FL) or Myc-hTERT fragments (amino acid numbers are indicated). FLAG-hEST1A was immunoprecipitated using α-FLAG antibodies. Upon immunoprecipitation and five washes, samples were loaded on 4–20% gradient SDS–polyacrylamide gels. The presence of Myc-hTERT fragments was determined by western blotting with α-Myc antibodies and the immunoprecipitation efficiency was determined by western blotting with α-FLAG antibodies. The presence of the polypeptides is indicated by black arrows whereas white arrows indicate absence of the polypeptides.