Table 1.
Real-time PCR analysis: sequence of the oligonucleotide pairs, annealing temperature, and product size
| Genes | Species | Acc number | Primer pairs | Ta(°C)a | Size(bp)b |
|---|---|---|---|---|---|
| GAPDH | Murine | BC083079 | F CATGGCCTTCCGTGTTCCTA R TGCCTGCTTCACCACCTTCT | 60 | 105 |
| Human | NM_002046 | F AGCTCACTGGCATGGCCTTC R ACGCCTGCTTCACCACCTTC | 60 | 117 | |
| HO-1 | Murine | NM_010442 | F GGAGATAGAGCGCAACAAGC R CCATACCAGAAGGCCATGTC | 60 | 105 |
| Human | NM_002133 | F CCAGTGCCACCAAGTTCAAG R CAGCTCCTGCAACTCCTCAA | 60 | 143 | |
| Waf1/p21 | Murine | NM_007669 | F TTGTCGCTGTCTTGCACTCT R ATCTGCGCTTGGAGTGATAG | 60 | 124 |
aTa: annealing temperature; bSize of the amplified fragment.