Table 1.

Globally fit kinetic parameters for AZT incorporation

Kd (μM)	k2 (s−1)	k-2 (s−1)	k3 (s−1)
230°±°25	0.38°±°0.04	0.22 °±°0.02	0.0009°±°0.00004

Rate constants and error estimates were obtained by simultaneously fitting presteady state and steady-state kinetic data (Figures 1A and 5B) to the minimal model shown in Scheme 1 as described in the Methods section.