Figure 6.

Disrupted expression of Inpp5f_v2 and Inpp5f_v3 in Dnmt1 and Dnmt3l mutants. (A) Qualitative RT-PCR expression assay for Inpp5f_v3 in E8 embryos carrying null mutations in Dnmt1. Inpp5f_v3 was amplified for 34 cycles, Gapdh was amplified for 30 cycles. Parallel amplifications were performed using cDNA generated in the presence (+RT) or absence (–RT) of reverse transcriptase. (B) Allele-specific CpG methylation at the two promoter regions in E8.5 embryos derived from Dnmt3l^−/– mothers (on a Mus musculus domesticus background) and wild-type cast fathers. (C) Allele-specific RT-PCR sequencing assay for Inpp5f_v2 in E8.5 embryos derived from mothers carrying homozygous null mutations in the Dnmt3l gene, on a Mus domesticus background. The maternal strain or genotype is given first in the hybrid crosses.