Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 4;35(20):6740–6749. doi: 10.1093/nar/gkm745

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — All tRNA^Thr isoacceptors are substrate for aminoacylation in vivo. (A) Total RNA from T. brucei was extracted under acidic conditions and aminoacylation levels were correlated to editing levels by the oxopap assay as described. (B) Independent clones derived from the ‘+aa’ reaction (in A) were used in the OXOPAP assay (Materials and Methods section) and analyzed by sequencing. In all cases both edited and unedited species are substrates for aminoacylation in vivo. (C) Total RNA from the same fraction as above was also separated by acid denaturing polyacrylamide electrophoresis and probed with radioactive oligonucleotides specific for either tRNA^ThrAGU (top panel) or tRNA^ThrCGU/UGU. The probe used does not discriminate between the CGU and UGU isoacceptors. ‘−aa’ refers to a control reaction in which the RNA was deacylated by incubating under basic conditions prior to analyses. ‘+aa’ refers to the aminoacylated fractions purified and kept under acid pH.