Table 1.
Plasmids used in this study
| Name | Relevant feature(s) | Reference |
|---|---|---|
| pACYC177 | Cloning vector (AmpR, KanR) | (90) |
| pBAD24 | Arabinose inducible araBAD promoter, araC, AmpR, ColE1 ori | (68) |
| pBR322 | Cloning vector (AmpR, TetR) | (91) |
| pDK200 | wt pvuIIC gene with its own promoter, ▵pvuIIM, ▵pvuIIR, TetR, p15A ori | (43) |
| pDK201 | As pDK201, but pvuIIC-Esp19 mutation | (43) |
| pDK178 (WWWW) | Transcriptional fusion of pvuIIC promoter including C-boxes with the symmetrical core - WWWW (GATCcat AGCTtgtaGACTcaaAGCT) to the cat gene in pKK232-8 | D. Knowle, unpublished |
| pDK435 | Transcriptional fusion of pvuIIC promoter including wt C-boxes (positions −93 to +88) in front of promotorless lacZ reporter gene in pKK232-8, TetR, p15A ori | D. Knowle, unpublished |
| pIM1 | pvuIIC under control of araBAD promoter in pBAD24, AmpR, ColE1 ori | This study |
| pIM2 | Kan cassette inserted to break bla gene in pIM1, KanR, ColE1 ori | This study |
| pIM4 | As pIM2 but p15A ori | This study |
| pIM6 | Nonrepression mutant, as pPvuRM3.4, but C-box (GACTcatAGTCtgtaGACTcaaGATC) | This study |
| pIM8 | As pDK435, but symmetrized C-box, as for pDK178 | This study |
| pIM9 | As pDK435, but C-box sequence as for pIM6 | This study |
| pKK232-8 | Promotorless cat reporter gene | (66) |
| pMal-c2x | Vector for purification of protein fused to malE (maltose-binding protein) | New England Biolabs |
| pMal.CPvII | Translational fusion of pvuIIC::malE in pMal-c2x | This study |
| pPvuRM3.4 | wt PvuII R-M system in pBR322 backbone | (4) |
| pPvuM1.9-ACYC | As pPvuM1.9 (M+R-C-), but p15A ori | (4) |
| pRWWW | As pDK178, C-box–RWWW (TCAGcatAGTCtgtaGACTcaaAGCT) | D. Knowle, unpublished |
| pWWWR | As pDK178, C-box–WWWR (GACTcatAGTCtgtaGACTcaaCTGA) | D. Knowle, unpublished |
| pWWRW | As pDK178, C-box–WWRW (GACTcatAGTCtgtaTCAGcaaAGTC) | D. Knowle, unpublished |
| pWRWW | As pDK178, C-box–WRWW (GACTcatCTGAtgtaGACTcaaAGCT) | D. Knowle, unpublished |
| UL3 (WWRR) | As pDK178, C-box–WWRR (GACTcatAGTCtgtaTCAGcaaCTGA) | This study |
| UL4 (RRWW) | As pDK178, C-box–RRWW (TCAGcatCTGAtgtaGACTcaaAGCT) | This study |