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. 2007 Oct 5;35(20):6788–6797. doi: 10.1093/nar/gkm782

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Effect of Sta1 on T6 and sso0777 promoters. The in vitro transcription assay was performed in the presence of increasing concentrations of Sta1 (0, 50, 250, 500, 1000, 1500 and 2500 ng). The products of the in vitro transcription of promoters indicated were detected by primer extension analysis and electrophored on a 12% denaturing polyacrylamide gel. RNAP : TFB-1 : TBP concentrations used were 40 : 300 : 300 (nM). Percentage of transcription referred to the first lane (no Sta1) is shown under each panel.