Table 1.

Walking-PCR cycling program

Round	Cycles	Temperature	Time	Action
		94°C	4 min	Primary denaturation
		94°C	30 s	Specific primer extension; ssDNA synthesis
1	30	56°C	30 s
		72°C	3 min
		94°C	30 s	Unspecific binding of primer; dsDNA synthesis
2	1	40°C	30 s
		72°C	3 min
		94°C	30 s	Specific exponential amplification
3	30	56°C	30 s
		72°C	3 min
		72°C	10 min	Final elongation