Figure 5.

Sinefungin phenotype of sfr sam3Δ strains. sam3Δ cells transformed with a CEN TRP1 vector containing the genes specified at left were grown in Trp⁻ medium at 30°C until A₆₀₀ reached ∼0.7. The cells were harvested by centrifugation and suspended in water. Serial 10-fold dilutions were prepared and aliquots (2 µl) were spotted on an unsupplemented Trp⁻ agar plate (‘no drug’) and on Trp⁻ plates onto which 150 µl of a 0.5 mM or 2 mM sinefungin solution had been applied and spread. The plates were photographed after incubation for 3 days at 30°C.