Figure 6.

Sam3 overexpression sensitizes yeast to sinefungin inhibition. Wild-type SAM3 cells were transformed with 2 µ SAM3 or 2 µ plasmids and grown in His⁻ medium at 30°C until A₆₀₀ reached ∼0.7. The cells were harvested by centrifugation and suspended in water. Serial 10-fold dilutions were prepared and aliquots (2 µl) were spotted on an unsupplemented His⁻ agar plate (‘no drug’) and on His⁻ plates onto which 150 µl of a 1.6 µM or 3.1 µM sinefungin solution had been applied and spread. If the applied drug is evenly distributed through the depth of the agar plate, these doses correspond to net sinefungin concentrations of about 10 and 19 nM, respectively. The plates were photographed after incubation for 3 days at 30°C.