Table 2.

Rates of incorporation of dNTPs opposite an abasic lesion on either the DNA or RNA template with the standing start primer (Figure 1) by HIV-1 RT

Template backbone	X	dTP	Vmax (nM·min−1)	KM (μM)	kcat (min−1)	kcat/KM (μM−1·min−1)	krela
RNA	U	A	1.75 ± 0.08	13.47 ± 5.99	0.089 ± 0.004	6.57×10−3	13.5
	rAS	A	3.61 ± 0.40	54.84 ± 10.64	0.183 ± 0.020	3.33 × 10−3	6.7
	rAS	G	7.19 ± 0.92	80.62 ± 15.87	0.182 ± 0.023	2.26 × 10−3	4.5
	rAS	C	4.83 ± 0.15	148.75 ± 12.05	0.082 ± 0.003	0.55 × 10−3	1.1
	rAS	T	8.05 ± 2.17	275.21 ± 75.95	0.136 ± 0.037	0.50 × 10−3	1
DNA	T	A	5.47 ± 1.04	2.88 ± 0.67	1.385 ± 0.263	4.81 × 10−1	1551
	dAS	A	6.98 ± 0.24	25.86 ± 1.90	1.768 ± 0.060	6.84 × 10−2	221
	dAS	G	7.16 ± 0.81	68.46 ± 14.88	0.725 ± 0.083	1.06 × 10−2	34
	dAS	C	3.39 ± 0.31	185.77 ± 18.89	0.057 ± 0.005	0.31 × 10−3	1
	dAS	T	5.93 ± 1.76	349.88 ± 150.86	0.150 ± 0.044	0.43 × 10−3	1.4

^ak_rel = (k_cat/K_M)_N/(k_cat/K_M)_min.