Figure 6.
Histogram showing residual (%) UDP-MurNAc-hexapeptide synthesis in the AlaRS-FemXWv coupled assay with derivatives of tRNAAla (76-nt) harboring all possible combinations in base pairs G1–C72 (A), G2–C71 (B), G3–U70 (C) and G4–C69 (D). The standard assay was performed with AlaRS (800 nM), FemXWv (500 nM), l-[14C]Ala (50 µM) and the different tRNAs (ca. 5 µM). The reaction was incubated for 2 h at 30°C. Under these conditions, total l-[14C]Ala (0.5 nmol) was incorporated into UDP-MurNAc-hexapeptide when RNA with the wild type sequence was used (100%, indicated by a star).