Table 1.
DNA sequences consistent with the consensus ARE motifs located within 10 Kb upstream of the HMOX1 TSS and 1 Kb upstream of TXNRD1 TSS
| Postulated antioxidant response elements of the HMOX1 and TXNRD1 genes | ||
|---|---|---|
| PCR primer location for ChIP (bp) | Motif sequence | Motif location (bp) |
| Consensus motif: GCnnnRTCAY or CGnnnYAGTR | ||
| HMOX1 | ||
| 9069 | GTGACagaGC | 9491 |
| GCtgaGTCAC | 9066 | |
| GCtaaGTCAC | 9037 | |
| GCtgaGTCAC | 9008 | |
| GCtgaGTCAC | 8979 | |
| 7232 | GCcttGTCAC | 7104 |
| 6060 | GCagaATCAT | 6008 |
| GCtgaATCAT | 5967 | |
| 3928 | GCtgcGTCAT | 3992 |
| GCtgaGTCAC | 3928 | |
| 1319 | GCgtgGTCAC | 107 |
| 148 | GCaaaATCAC | 200 |
| TXNRD1 | ||
| 91 & 36 | GCtttGTCAT | 19 |
PCR primer and motif positions correspond to the most distant 5′ nucleotide from the TSS on the forward strand. Sequences were considered candidate ARE motifs regardless of orientation or strand.