Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 25;35(21):7313–7323. doi: 10.1093/nar/gkm726

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-com

PMC Copyright notice

Figure 6. — Automethylated G9a binds to HP1 family of proteins. (A) GST pull-down assay with G9afl (automethylated) or G9amut containing K239A (unmethylated) enzyme. The top panel shows ponceau stain of the western blotted membrane demonstrating similar loading of GST-HP1α, GST-HP1β and GST-HP1γ. The blot was probed with anti-G9a as indicated. The molecular weight markers are on the left. Immunofluorescence detection of co-localization events are as follows (B) GFP-G9a and anti-HP1α; (C) GFP-G9amut and anti-HP1α; (D) GFP-G9a and anti-HP1γ; (E) GFP-G9amut and anti-HP1γ. Both HP1α and HP1γ are shown in red.