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. 2007 Nov 5;35(21):e142. doi: 10.1093/nar/gkm932

Figure 4.

Figure 4.

Two-hybrid interaction between pVHL and the candidate substrate, DGKι. (a) Schematic representation of full-length DGKι and the constructs used in two-hybrid assays. Key domains and sequence motifs are indicated at the top, and the two LxxLAP motifs are indicated at the bottom of the bars. (b) ELB-, ELC- and LxxLAP motif-dependent interaction between pVHL and DGKι. Two-hybrid interactions between BD-pVHL and the indicated AD-DGKι and AD-HIF1α constructs were assayed as in Figure 2a. (c) Expression levels of AD-DGKι constructs. Protein samples were prepared as in Figure 2b–f. Shown are representative samples taken from CG1945BC cells expressing the indicated DGKι constructs. Expression levels were determined by western blot (WB) using Gal4-AD antibody. Asterisks indicate bands of the different DGKι constructs.