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. 2007 Nov 5;35(21):e142. doi: 10.1093/nar/gkm932

Figure 6.

Figure 6.

pVHL and DGKι interact in human cells. Lysates (Input) of human 293T cells overexpressing HA-tagged DGKι in the absence (–) or presence of the indicated FLAG-tagged pVHL isoform were subjected to immunoprecipitation using anti-FLAG antibody (IP: FLAG). Immunoprecipitated pVHL and DGKι were detected by western blot (WB) using anti-FLAG and anti-DGKι antibodies as indicated. The asterisk marks the light chain of the anti-FLAG antibody used for immunoprecipitation.