Table 2.

pVHL interactors identified in the yeast two-hybrid screen

Prey	Longest fragment founda	Number of hits	PHD3 requirementb	LxxLAP motifc
Elongin C	aa 4–112 (CT)	26	No	No
Elongin B	aa 2–118 (CT)	10	No	No
HIF1α	aa 232–439,	6	Yes	Yes
	aa 537–777
HIF2α	aa 331–537	1	Yes	Yes
DGKι	aa 668–910	2	Yes	Yes
KIF2C	aa 558–681	1	Yes	Yes
THO1	aa 578–657 (CT)	1	Yes	(Yes)
Calpain 7	aa 536–679	8	Yes	(Yes)
YY1AP1	aa 604–739 (CT)	20	Yes	No
RBP-MS/type 3	aa 23–201	1	Yes	No
RB1CC1	aa 1407–1594 (CT)	1	Yes	No
FKBP38	aa 97–344	1	Yes	No

^aAmino acid residues as determined by sequencing of the prey plasmid insert from the 5′ end; (CT), residue number denotes the authentic C-terminus of the protein; otherwise, the second residue number indicates the end of the sequenced region, not the 3′ end of the prey plasmid insert. Because an oligo(dT)-primed cDNA library was used, most inserts include the authentic 3′ end of the coding sequence and some 3′ untranslated region of the cDNA.

^bAs tested by co-transformation of the prey plasmid with pBridge-pVHL/empty.

^cBrackets indicate presence of divergent motifs that may still be recognized by PHDs (32).