Table 1.

Detection of 48 alternative cassette exons from a pool of cDNAs by different RT-PCR methods

Method of PCR	Flanking PCR		Semi-nested PCR
Method of RT priming	Oligo-dT	Random hexamer	Oligo-dT	Random hexamer	Exon specific
Number of alternatives detected	7/48 (14.6%)	20/48 (41.7%)	29/48 (60.4%)	42/48 (87.5%)	47/48 (97.9%)

Note: The sensitivity for each method is shown in parentheses.