Figure 3.
Competition for DNA polymerase between wild-type and chimerical TPs. The assay of formation of TP-dAMP initiation product by the wild-type ϕ29 and GA-1 DNA polymerase/TP heterodimers was performed in the presence of increasing amounts of chimeras N-ϕ (open circles) and N-G (filled circles), respectively (the reaction conditions are described under Materials and Methods section). Reactions were started by adding 1 mM MnCl2 and, after incubation for 10 min at 30°C, reactions were stopped and analysed as indicated for the protein-primed initiation assay. The TP-dAMP formed in the different competition conditions relative to that formed in the absence of competition (100%), as well as the theoretical inhibition profile (open squares) that would be obtained if chimerical TPs showed a wild-type interaction with the corresponding DNA polymerase are indicated. Graphic is representative of three independent experiments.