Figure 4.

In vitro TP-DNA replication. The assays were carried out in the presence of 1 mM MnCl₂, 60 nM of the indicated DNA polymerase, 120 nM of wild-type or chimerical TP and 80 µM of the four dNTPs. After incubation for 8 min (wild-type TPs) and 30 min (with chimerical TPs) at 30°C, samples were stopped and processed as described in Materials and Methods section. The labelled DNA was denatured and subjected to electrophoresis in alkaline agarose gels to determine the lengths of the synthesized DNAs. The position of unit length TP-DNA is indicated.