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. 2007 Oct 25;35(21):7336–7347. doi: 10.1093/nar/gkm863

Figure 7.

Figure 7.

Reversibility of the ncmtRNA expression. (a) Human lymphocytes were incubated without (white bars) or with PHA (hatched bars) for 24, 48 and 72 h. At each time period, the cells were incubated with BrdU for 16 h and the incorporation of the nucleoside was determined by ELISA (see Materials and Methods section). The incorporation of BrdU is expressed as the absorbance at 450 nm. (b) About 100 000 lymphocytes incubated without (−PHA) or with PHA (+PHA) for 72 h were subjected to immunocytochemistry to determine the expression of Ki-67, PCNA and phospho-histone H3 (PO4-H3), and to ISH with probe 13 to determine the expression of the ncmtRNA (×40). (c) PHA-stimulated lymphocytes for 72 h were hybridized with probe 13 (ncmtRNA) or with the corresponding sense control probe (Control sense) (×20). (d) DU145 cells were incubated twice with aphidicolin (see Methods section) and subjected to ISH together with a control culture. The blocked cells were changed to normal medium for 48 h and then subjected to ISH (Recovery) (×40).